Many DNA extraction methods can be laborious and time consuming, involving phenol extraction, overnight incubations often combined with column purification. MyTaq Extract-PCR Kit offers a rapid and easy alternative for the extraction and amplification of DNA from a variety of tissue types, particularly solid tissues such as mouse tail (fig. 1) or mouse ear. The DNA extractions are performed in a single-tube, without the need for multiple washing steps, greatly reducing the risk of sample loss and contamination.
The extracted DNA is amplified using MyTaq HS Mix, the latest generation of very high performance polymerase mastermixes unique to Bioline, powered by antibody mediated hot-start to reduce non-specific amplification. The advanced formulation of MyTaq HS Mix allows fast cycling conditions to be used, greatly reducing the reaction time without compromising PCR specificity or yield (fig. 2).
Together, the single tube lysis protocol and MyTaq HS Mix maximizes sensitivity, while minimizing contamination risk, significantly reduces reaction times as well as delivering improved success rates in protocols such as mouse DNA characterization.