The SensiFAST SYBR Hi-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time instruments. A combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensures that the SensiFAST SYBR Hi-ROX Kit produces fast, highly-specific and ultra-sensitive real-time PCR.
The SensiFAST SYBR Hi-ROX Kit contains premixed ROX for optional use.
** Subject to Bioline review. **Order must be placed within 30 days of review submission.**50% off list price on first pack ordered.
I used a trial of the SensiFAST SYBR Hi-ROX Kit for my qPCR requirements. The product gave nice consistent results, of a quality slightly higher than a competitors. The fact that it has all dNTPs, MgCl2 etc in one solution means I am far less likely to make mistakes and allows faster troubleshooting.
Joshua Brian - Victoria University of Wellington - SBS
"We have recently sampled the Sensifast Hi-ROX SYBR q-PCR kit, and have been really pleased with the results.
The kit was run in parallel with the PerfeCTta SYBR Green supermix q-PCR kit, which we have been using in the lab for some time. q-PCR was carried out on treatment vs non-treatment cDNA samples, with two or three housekeeping genes for normalisation (results below). With threshold adjustment, dCt trends between samples were similar between kits, and Sensifast Hi-ROX SYBR showed less background noise (blue curves). Due to both it’s overall results and competitiveness in pricing , we have already placed an order for a kit."
Jessica Fitch - University of Auckland - SBS
Features and Benefits
• Rapid - hot-start capability saves time and reduces primer-dimer formation
• Unique buffer chemistry - for highest specificity
• Broad dynamic range – For high sensitivity
• Simple and reproducible - just add primers and template
ABI 7000, 7300, 7700, 7900, 7900HT, 7900HT FAST, StepOne™, StepOne™ Plus (See product selection table) each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.
BIO-92002: 200 x 20µl Reactions: 2 x 1ml
BIO-92005: 500 x 20µl Reactions: 5 x 1ml
BIO-92020: 2000 x 20µl Reactions: 20 x 1ml
2x Reaction mix consisting of:
• Heat-activated DNA polymerase
• Ultra-pure dNTPs
• SYBR® Green I
The kit size is based on a 20µl final reaction volume.
6 months at -20°C. Avoid exposure of the SYBR® Green I to light.
SensiFAST is a trademark of Bioline Reagents Ltd.
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