Real-Time PCR Mixes

Bioline SensiFAST™ SYBR Lo-ROX Kit

Bioline SensiFAST™ SYBR Lo-ROX Kit

Features

The SensiFAST SYBR Lo-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time instruments. A combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensures that the SensiFAST SYBR Lo-ROX Kit produces fast, highly-specific and ultra-sensitive real-time PCR.

The SensiFAST SYBR Lo-ROX Kit contains premixed ROX for optional use.

** Subject to Bioline review. **Order must be placed within 30 days of review submission. **50% off list price on first pack ordered.

To request your sample call 09 273 2080 or email marketing@totallab.co.nz

For more information, or to submit your review.

http://bioline.com/au/review

Customer Review

Product: SensiFAST™ SYBR® Lo-ROX Kit
We trialled the SensiFast SYBR LoRox on a QuantStudio real time PCR machine using primers supplied by Qiagen. In a side-by-side comparison with two other SYBR green master mixes from different suppliers, we found SensiFast gave us lower cT values and lower variation between replicates. We also found we could detect the presence of a less abundant transcript in samples when we had used SensiFast, but not when we had used our two other SYBR master mixes. 
Raewyn Poulsen, University of Auckland, Faculty of Medicine, New Zealand

 

SensiFAST™ SYBR Lo-ROX Kit Pack Size:

Delivery time: 2 Weeks
Delivery times may vary
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Features and Benefits
• Rapid - hot-start capability saves time and reduces primer-dimer formation
• Accurate quantification - from low copy targets
• Broad dynamic range – For high sensitivity
• Flexible - compatible with many standard and fast cycling instruments

Instrument Compatibility
ABI 7500, 7500 FAST, ViiA7™, Stratagene (Agilent) Mx4000™, Mx3000P™, Mx3005P™ (See product selection table), as well as other instruments that do not require the use of a passive reference.

Volume
BIO-94002: 250 x 20µl Reactions: 2 x 1ml
BIO-94005: 500 x 20µl Reactions: 5 x 1ml
BIO-94020: 2000 x 20µl Reactions: 20 x 1ml

Components
2x Reaction mix consisting of:
• Heat-activated DNA polymerase
• Ultra-pure dNTPs
• MgCl2
• ROX
• SYBR® Green I

 Kit Size
The kit size is based on a 20µl final reaction volume.

Storage Conditions
6 months at -20°C. Avoid exposure of the SYBR® Green I to light.

Notes
SensiFAST is a trademark of Bioline Reagents Ltd.

1. Jacob, F., et al. PLoS ONE 7(2), e31885. doi:10.1371/journal.pone.0031885 (2012).

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