SensiFAST HRM uses the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure that the SensiFAST HRM Kit delivers fast, reproducible, highly-specific and ultra-sensitive HRM analysis.
ABI 7300, 7500 FAST, Qiagen Rotor-Gene™ 6000, Eppendorf Mastercycler® ep realplex, Illumina® Eco™, Roche LightCycler®; 96 and 480, Bio-Rad Opticon®, CFX96™, CFX384™, Idaho LightScanner® 32 (LS32™), Takara Thermal Cycler Dice (TP800) (See product selection table).
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High Resolution Melt (HRM) curve analysis is a post-PCR analysis method of characterizing nucleic acid samples based on DNA strand dissociation behaviour during transition from double-stranded DNA to single-stranded DNA with increasing temperature. It uses intercalating dyes, highly accurate melt curves and the application of specific statistical analyses.
SensiFAST HRM has been developed using the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure that the SensiFAST HRM Kit delivers fast, reproducible, highly-specific and ultra-sensitive HRM analysis, ideal for small and medium-sized genotyping projects.
The combination of our SensiFAST DNA Polymerase, buffer and EvaGreen® dye enables amplification and discrimination of even the most challenging sequence differences, such asclass 4 SNPs (Fig. 1) without sequence preference. Main applications of HRM include Single Nucleotide Polymorphisms (SNPs) genotyping, epigenetics (DNA methylation analysis), zygosity testing (DNA mapping and DNA fingerprinting) and gene scanning (search for the presence of unknown variations in PCR amplicons).
200 x 20µl reactions
500 x 20µl reactions
2000 x 20µl reactions
SensiFAST HRM mix (2x)
2 x 1ml
5 x 1ml
20 x 1ml
IO-32002: 200 x 20µl Reactions: 2 x 1ml
BIO-32005: 500 x 20µl Reactions: 5 x 1ml
BIO-32020: 2000 x 20µl Reactions: 20 x 1ml
Storage & Stability
All kit components should be stored at -20°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label. Avoid exposure of the EvaGreen to light.
SensiFAST HRM Kit is shipped on dry/blue ice.
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