• Rapid - hot start capability saves time and reduces primer-dimer formation
• Unique buffer chemistry - for highest specificity and sensitivity
• Accurate quantification - from low copy targets
• Sensitive – unique buffer chemistry for highest specificity
Roche LightCycler® 480 platform, Bio-Rad Opticon®, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, 6000, Eppendorf Mastercycler® ep realplex and Techne Quantica®. (See product selection table).
The SensiFAST™ SYBR® No-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system, ensures that SensiFAST SYBR® No-ROX One-Step Kit produces fast, highly-specific and ultra-sensitive one-step RT-qPCR.
The SensiFAST SYBR® No-ROX One-Step Kit consists of a 2x SensiFAST SYBR® One-Step mix, as well as separate reverse transcriptase and RiboSafe RNase Inhibitor.
BIO-72001: 100 x 20µl Reactions: 1 x 1ml
BIO-72005: 500 x 20µl Reactions: 5 x 1ml
2x Reaction mix consisting of:
•Heat-activated DNA polymerase
Plus separate vials of:
The kit size is based on a 20µl final reaction volume.
6 months at -20°C. Avoid exposure of the SYBR® Green I to light.
SensiFAST is a trademark of BiolineReagents Ltd.
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