Real-Time PCR Mixes

Bioline SensiFAST™ SYBR No-ROX One-Step Kit

Bioline SensiFAST™ SYBR No-ROX One-Step Kit


• Rapid - hot start capability saves time and reduces primer-dimer formation
• Unique buffer chemistry - for highest specificity and sensitivity
• Accurate quantification - from low copy targets
• Sensitive – unique buffer chemistry for highest specificity

** Order must be placed within 30 days of review submission. 30% off list price for the smallest pack size. Subject to Bioline review.

To request your sample call 09 273 2080 or email

For more information, or to submit your review.

SensiFAST™ SYBR No-Rox One Step-Kit Pack Size:

Delivery time: 2 Weeks
Delivery times may vary
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Instrument Compatibility
Roche LightCycler® 480 platform, Bio-Rad Opticon®, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, 6000, Eppendorf Mastercycler® ep realplex and Techne Quantica®. (See product selection table).

The SensiFAST™ SYBR® No-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system, ensures that SensiFAST SYBR® No-ROX One-Step Kit produces fast, highly-specific and ultra-sensitive one-step RT-qPCR.

The SensiFAST SYBR® No-ROX One-Step Kit consists of a 2x SensiFAST SYBR® One-Step mix, as well as separate reverse transcriptase and RiboSafe RNase Inhibitor.

BIO-72001: 100 x 20µl Reactions: 1 x 1ml
BIO-72005: 500 x 20µl Reactions: 5 x 1ml

2x Reaction mix consisting of:
•Heat-activated DNA polymerase
•Ultra-pure dNTPs
•SYBR®Green I

Plus separate vials of:
•Reverse transcriptase
•RNase inhibitor
•DEPC-treated water

Kit Size
The kit size is based on a 20µl final reaction volume.

Storage Conditions
6 months at -20°C. Avoid exposure of the SYBR® Green I to light.

SensiFAST is a trademark of BiolineReagents Ltd.

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