• Rapid - hot start capability saves time and reduces primer-dimer formation
• Unique buffer chemistry - for highest specificity and sensitivity
• Accurate quantification - from low copy targets
• Sensitive – unique buffer chemistry for highest specificity
ABI 7000, 7300, 7700, 7900, 7900HT, StepOne™, StepOne™ Plus, (See product selection table), each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.
The SensiFAST SYBR® Hi-ROX One-Step Kit has been developed for fast RT-qPCR and has been validated on several commonly used real-time instruments. The SensiFAST SYBR® Hi-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The SensiFAST SYBR® Hi-ROX One-Step Kit uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR (Fig. 1).
The SensiFAST SYBR® Hi-ROX One-Step Kit consists of a 2x SensiFAST SYBR® One-Step mix, which contains ROX for optional use, plus separate reverse transcriptase and RiboSafe RNase Inhibitor.
BIO-73001: 100 x 20µl Reactions: 1 x 1ml
BIO-73005: 500 x 20µl Reactions: 5 x 1ml
2x Reaction mix consisting of:
• Heat-activated DNA polymerase
• Ultra-pure dNTPs
• SYBR® Green I
Plus separate vials of:
• Reverse transcriptase
• RNase inhibitor
• DEPC-treated water
The kit size is based on a 20µl final reaction volume.
6 months at -20°C. Avoid exposure of the SYBR® Green I to light.
SensiFAST is a trademark of Bioline Reagents Ltd.
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Bergland A.O. et al. PLoS Genet. 8(4): e1002631(2012)
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