Real-Time PCR Mixes

Bioline SensiFAST™ SYBR Hi-ROX One-Step Kit

Bioline SensiFAST™ SYBR Hi-ROX One-Step Kit

Features

• Rapid - hot start capability saves time and reduces primer-dimer formation
• Unique buffer chemistry - for highest specificity and sensitivity
• Accurate quantification - from low copy targets
• Sensitive – unique buffer chemistry for highest specificity

** Order must be placed within 30 days of review submission. 30% off list price for the smallest pack size. Subject to Bioline review.

To request your sample call 09 273 2080 or email marketing@totallab.co.nz

For more information, or to submit your review.

http://bioline.com/au/review

SensiFAST™ SYBR Hi-ROX One-Step Kit Pack Size:

Delivery time: 2 Weeks
Delivery times may vary
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Instrument Compatibility
ABI 7000, 7300, 7700, 7900, 7900HT, StepOne™, StepOne™ Plus, (See product selection table), each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.

Description
The SensiFAST SYBR® Hi-ROX One-Step Kit has been developed for fast RT-qPCR and has been validated on several commonly used real-time instruments. The SensiFAST SYBR® Hi-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The SensiFAST SYBR® Hi-ROX One-Step Kit uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR (Fig. 1).

The SensiFAST SYBR® Hi-ROX One-Step Kit consists of a 2x SensiFAST SYBR® One-Step mix, which contains ROX for optional use, plus separate reverse transcriptase and RiboSafe RNase Inhibitor.

Volume
BIO-73001: 100 x 20µl Reactions: 1 x 1ml
BIO-73005: 500 x 20µl Reactions: 5 x 1ml

Components
2x Reaction mix consisting of:
• Heat-activated DNA polymerase
• Ultra-pure dNTPs
• MgCl2
• ROX
• SYBR® Green I

Plus separate vials of:
• Reverse transcriptase
• RNase inhibitor
• DEPC-treated water

Kit Size
The kit size is based on a 20µl final reaction volume.

Storage Conditions
6 months at -20°C. Avoid exposure of the SYBR® Green I to light.

Notes
SensiFAST is a trademark of Bioline Reagents Ltd.

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Bergland A.O. et al. PLoS Genet. 8(4): e1002631(2012)

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