Real-Time PCR Mixes

Bioline SensiFAST™ SYBR Lo-ROX One-Step Kit

Bioline SensiFAST™ SYBR Lo-ROX One-Step Kit


• Rapid - hot start capability saves time and reduces primer-dimer formation
• Reproducible – Optimized reverse-transcription and amplification buffer
• Accurate quantification - of gene expression over a broad dynamic range
• Sensitive – unique buffer chemistry for highest specificity

** Order must be placed within 30 days of review submission. 30% off list price for the smallest pack size. Subject to Bioline review.

To request your sample call 09 273 2080 or email

For more information, or to submit your review.

SensiFAST™ SYBR Lo-ROX One-Step Kit Pack Size:

Delivery time: 2 Weeks
Delivery times may vary
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Instrument Compatibility
ABI 7500, 7500 FAST, Stratagene (Agilent) Mx4000™, Mx3000P™, Mx3005P™, (See product selection table), as well as other instruments that do not require the use of a passive reference.

The SensiFAST SYBR® Lo-ROX One-Step Kit has been developed for fast RT-qPCR and has been validated on many commonly used real-time instruments. The kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The SensiFAST SYBR® Lo-ROX One-Step Kit uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR (Fig. 1).

The SensiFAST SYBR® Low-ROX One-Step Kit consists of a 2x SensiFAST SYBR® One-Step mix, which contains ROX for optional use, separate reverse transcriptase and RiboSafe RNase Inhibitor.

BIO-74001: 100 x 20µl Reactions: 1 x 1ml
BIO-74005: 500 x 20µl Reactions: 5 x 1ml

2x Reaction mix consisting of:
• Heat-activated DNA polymerase
• Ultra-pure dNTPs
• MgCl2
• SYBR® Green I

Plus separate vials of:
• Reverse transcriptase
• RNase inhibitor
• DEPC-treated water

Kit Size
The kit size is based on a 20µl final reaction volume.

Storage Conditions
6 months at -20°C. Avoid exposure of the SYBR® Green I to light.

SensiFAST is a trademark of BiolineReagents Ltd.

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