• Rapid - hot start capability saves time and reduces primer-dimer formation
• Reproducible – Optimized reverse-transcription and amplification buffer
• Accurate quantification - of gene expression over a broad dynamic range
• Sensitive – unique buffer chemistry for highest specificity
ABI 7500, 7500 FAST, Stratagene (Agilent) Mx4000™, Mx3000P™, Mx3005P™, (See product selection table), as well as other instruments that do not require the use of a passive reference.
The SensiFAST SYBR® Lo-ROX One-Step Kit has been developed for fast RT-qPCR and has been validated on many commonly used real-time instruments. The kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The SensiFAST SYBR® Lo-ROX One-Step Kit uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR (Fig. 1).
The SensiFAST SYBR® Low-ROX One-Step Kit consists of a 2x SensiFAST SYBR® One-Step mix, which contains ROX for optional use, separate reverse transcriptase and RiboSafe RNase Inhibitor.
BIO-74001: 100 x 20µl Reactions: 1 x 1ml
BIO-74005: 500 x 20µl Reactions: 5 x 1ml
2x Reaction mix consisting of:
• Heat-activated DNA polymerase
• Ultra-pure dNTPs
• SYBR® Green I
Plus separate vials of:
• Reverse transcriptase
• RNase inhibitor
• DEPC-treated water
The kit size is based on a 20µl final reaction volume.
6 months at -20°C. Avoid exposure of the SYBR® Green I to light.
SensiFAST is a trademark of BiolineReagents Ltd.
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