Real-Time PCR Mixes

Bioline SensiFAST Probe No-ROX One-Step Kit


Rapid - Optimized for fast reverse transcription real-time PCR
Accurate quantification - For RNA from low copy targets
Sensitive – unique buffer chemistry for highest specificity
Flexible - Compatible with all fast cycling instruments

Instrument Compatibility

Roche LightCycler® 480 platform, Bio-Rad Opticon™, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, 6000, Eppendorf Mastercycler® ep realplex and Techne Quantica®. (See product selection table).


SensiFAST Probe No-ROX Pack Size:

Delivery time: 2 Weeks
Delivery times may vary
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The SensiFAST™ Probe No-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube (Fig. 1). The kit is designed for use with probe-detection technology (Fig. 2), including TaqMan®, Scorpions® and molecular beacon probes (Fig. 3). A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system ensures that SensiFAST Probe No-ROX One-Step Kit produces fast, highly-specific and ultra-sensitive one-step RT-qPCR.

The SensiFAST Probe No-ROX One-Step Kit consists of a 2x SensiFAST Probe No-ROX One-Step mix, separate reverse transcriptase and RiboSafe RNase Inhibitor.



BIO-76001: 100 x 20µl Reactions: 1 x 1ml
BIO-76005: 500 x 20µl Reactions: 5 x 1ml

2x Reaction mix consisting of:

Heat-activated DNA polymerase
Ultra-pure dNTPs

Plus separate vials of:
Reverse Transcriptase
RNase inhibitor
DEPC-treated water

Kit Size
The kit size is based on a 20µl final reaction volume.

Storage Conditions
6 months at -20°C.



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