Real-Time PCR Mixes

Bioline SensiFAST Probe No-ROX One-Step Kit

Bioline SensiFAST Probe No-ROX One-Step Kit


Rapid - Optimized for fast reverse transcription real-time PCR
Accurate quantification - For RNA from low copy targets
Sensitive – unique buffer chemistry for highest specificity
Flexible - Compatible with all fast cycling instruments

Instrument Compatibility

Roche LightCycler® 480 platform, Bio-Rad Opticon™, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, 6000, Eppendorf Mastercycler® ep realplex and Techne Quantica®. (See product selection table).

** Order must be placed within 30 days of review submission. 30% off list price for the smallest pack size. Subject to Bioline review.

To request your sample call 09 273 2080 or email

For more information, or to submit your review.

SensiFAST Probe No-ROX Pack Size:

Delivery time: 2 Weeks
Delivery times may vary
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The SensiFAST™ Probe No-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube (Fig. 1). The kit is designed for use with probe-detection technology (Fig. 2), including TaqMan®, Scorpions® and molecular beacon probes (Fig. 3). A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system ensures that SensiFAST Probe No-ROX One-Step Kit produces fast, highly-specific and ultra-sensitive one-step RT-qPCR.

The SensiFAST Probe No-ROX One-Step Kit consists of a 2x SensiFAST Probe No-ROX One-Step mix, separate reverse transcriptase and RiboSafe RNase Inhibitor.



BIO-76001: 100 x 20µl Reactions: 1 x 1ml
BIO-76005: 500 x 20µl Reactions: 5 x 1ml

2x Reaction mix consisting of:

Heat-activated DNA polymerase
Ultra-pure dNTPs

Plus separate vials of:
Reverse Transcriptase
RNase inhibitor
DEPC-treated water

Kit Size
The kit size is based on a 20µl final reaction volume.

Storage Conditions
6 months at -20°C.



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