Real-Time PCR Mixes

Bioline SensiFAST™ SYBR & Fluorescein Kit

Bioline SensiFAST™ SYBR & Fluorescein Kit


The SensiFAST SYBR & Fluorescein Kit uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive real-time PCR. SensiFAST SYBR & Fluorescein Kit has been optimized for use in SYBR Green-based real-time PCR.

The SensiFAST SYBR & Fluorescein Kit contains premixed Fluorescein for optional use.

** Order must be placed within 30 days of review submission. 30% off list price for the smallest pack size. Subject to Bioline review.

To request your sample call 09 273 2080 or email

For more information, or to submit your review.

SensiFAST™ SYBR & Fluorescein Kit Pack Size:

Delivery time: 2 Weeks
Delivery times may vary
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Features and Benefits

• Rapid - hot-start capability saves time, reduces primer-dimer formation
• Specific and sensitive - for detection of a wide range of template concentrations, even from hard to obtain and low copy number samples
• Fast protocols - can be used with the new generation of fast cyclers without compromising performance
• Flexible - suitable for block-based instruments where fluorescein is required to allow calculation of dynamic well factors

Instrument Compatibility
Bio-Rad iCycler®, iQ®5, MyiQ™ (See product selection table) each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of fluorescein.

BIO-96001: 250 x 20µl Reactions: 2 x 1ml
BIO-96005: 500 x 20µl Reactions: 5 x 1ml
BIO-96020: 2000 x 20µl Reactions: 20 x 1ml

2x Reaction mix consisting of:
• Heat-activated DNA polymerase
• Ultra-pure dNTPs
• MgCl2
• Fluorescein
• SYBR Green I

Kit Size
The kit size is based on a 20µl final reaction volume.

Storage Conditions
6 months at -20°C. Avoid exposure of the SYBR® Green I to light.

SensiFAST is a trademark of Bioline Reagents Ltd.

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