The SensiFAST™ Probe No-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on all commonly used real-time instruments. The kit has been formulated for use with probe-detection technology, including TaqMan®, Scorpions®and molecular beacon probes. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that the SensiFAST Probe Kit delivers fast, highly-specific and ultra-sensitive real-time PCR.
SensiFAST Probe is provided as a 2x mastermix containing all the components necessary for real-time PCR, including dNTPs, stabilizers and enhancers.
Features and Benefits
• Rapid - hot-start capability saves time and reduces primer-dimer formation
• Sensitive – perfect for hard to obtain and low copy number samples
• High performance - ideal for multiplexing and gene expression analysis
• Fast protocols - use with the new generation of fast cyclers
Roche LightCycler® 480 platform, Bio-Rad iCycler®, iQ®5, MyiQ™, Opticon™, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, 6000, Eppendorf Mastercycler®ep realplex, Illumina® Eco™, Takara Thermal Cycler Dice® (TP800) and Techne Quantica®. See product selection table).
BIO-86002: 250 x 20µl Reactions: 2 x 1ml
BIO-86005: 500 x 20µl Reactions: 5 x 1ml
BIO-86020: 2000 x 20µl Reactions: 20 x 1ml
2x Reaction mix consisting of:
• Heat-activated DNA polymerase
• Ultra-pure dNTPs
The kit size is based on a 20µl final reaction volume.
6 months at -20°C. Avoid exposure of the ROX™ to light.
SensiFAST is a trademark of Bioline Reagents Ltd.
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