Real-Time PCR Mixes

Bioline SensiMix II Probe No-ROX Kit

Features

The SensiMix™ II Probe No-ROX Kit is a high-performance reagent designed for greater sensitivity and specificity, it does not contain a passive reference and is suitable for those block-based and rotor-based machines where an internal reference is not required for normalization. The kit delivers superior performance in gene expression analysis and multiplexing and uses hot-start DNA polymerase to ensure fast, highly-specific and ultra-sensitive real-time PCR, generating reliable and highly reproducible data.

  • Superior PCR efficiency - with sequence specific probes
  • Higher specificity - perfect for low copy number samples
  • Simple and reproducible - ideal for multiplexing and gene expression analysis
  • Flexible - compatibility with all real-time PCR instruments

 

SensiMix II Probe No-Rox Kit Pack Size:

Delivery time: 2 Weeks
Delivery times may vary
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The SensiMix™ II Probe No-ROX Kit is a high-performance reagent designed for greater sensitivity and specificity, it does not contain a passive reference and is suitable for those block-based and rotor-based machines where an internal reference is not required for normalization. The kit has been formulated for use with probe-detection technology, including hydrolysis probes (e.g. TaqMan®), displacement probes (e.g. Molecular Beacons, Scorpion®) and hybridization probes (e.g. FRET), delivering superior performance in gene expression analysis (Fig. 1) and multiplexing (Fig. 2).

SensiMix II Probe No-ROX Kit employs a hot-start DNA polymerase for high PCR specificity and sensitivity. SensiMix possesses no polymerase activity during reaction set-up, thereby greatly reducing non-specific amplification, including primer-dimer formation, after pre-heating SensiMix becomes fully activated and in conjunction with specially optimized buffer chemistry, generates reliable and highly reproducible data on all real-time PCR instruments.

For expression analysis, SensiFAST cDNA Synthesis Kit is the perfect partner for SensiMix II Probe No-ROX Kit for very high-quality first strand cDNA synthesis and real-time results.

For ease-of-use, SensiMix II Probe is provided as a 2x mastermix containing all the components necessary for real-time PCR, including dNTPs and stabilizers.

 

Components

Reagent

500 x 50µl reactions

2000 x 50µl reactions

SensiMix™ II Probe No-ROX (2x)

10 x 1.25ml (12.5ml)

40 x 1.25ml (50ml)

50mM MgCl2

1 x 1ml

1 x 1ml

 

Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

 

Shipping conditions

SensiMix II Probe No-ROX Kit is shipped on dry/blue ice

 

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Instrument Compatibility

Roche LightCycler® 480 platform, LightCycler® Nano, Bio-Rad Opticon™, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q, Eppendorf Mastercycler®ep realplex, Illumina® Eco™, Takara Thermal Cycler Dice® (TP800), Analytik Jena qTOWER and Techne Quantica®, PrimeQ.

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