Real-Time PCR Mixes

Bioline SensiMix II Probe Hi-ROX Kit

Features

The SensiMix™ II Probe Hi-ROX Kit is a high-performance reagent designed for greater sensitivity and specificity on various real-time instruments in which a ROX signal can be used. The kit delivers superior performance in gene expression analysis and multiplexing and uses hot-start DNA polymerase to ensure fast, highly-specific and ultra-sensitive real-time PCR, generating reliable and highly reproducible data.

  • Superior PCR efficiency - with sequence specific probes
  • Higher specificity - perfect for low copy number samples
  • Simple and reproducible - ideal for multiplexing and gene expression analysis
  • Flexible - compatibility with all real-time PCR instruments

Delivery time: 2 Weeks
Delivery times may vary
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Description

The SensiMix™ II Probe Hi-ROX Kit is a high-performance reagent designed for greater sensitivity and specificity on various real-time instruments in which a ROX signal can be used. The kit has been formulated for use with probe-detection technology, including hydrolysis probes (e.g. TaqMan®), displacement probes (e.g. Molecular Beacons, Scorpion®) and hybridization probes (e.g. FRET), delivering superior performance in gene expression analysis (Fig. 1) and multiplexing (Fig. 2).

SensiMix II Probe Hi-ROX Kit employs a hot-start DNA polymerase for high PCR specificity and sensitivity. SensiMix possesses no polymerase activity during reaction set-up, thereby greatly reducing non-specific amplification, including primer-dimer formation, after pre-heating SensiMix becomes fully activated and in conjunction with specially optimized buffer chemistry, generates reliable and highly reproducible data on all real-time PCR instruments.

For expression analysis, SensiFAST cDNA Synthesis Kit is the perfect partner for SensiMix™ II Probe Hi-ROX Kit for very high-quality first strand cDNA synthesis and real-time results.

For ease-of-use, SensiMix II Probe is provided as a 2x mastermix containing all the components necessary for real-time PCR, including dNTPs and stabilizers.

 

Components

Reagent

500 x 50µl reactions

2000 x 50µl reactions

SensiMix™ II Probe Hi-ROX (2x)

10 x 1.25ml (12.5ml)

40 x 1.25ml (50ml)

50mM MgCl2

1 x 1ml

1 x 1ml

 

Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

 

Shipping conditions

SensiMix II Probe Hi-ROX Kit is shipped on dry/blue ice

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Instrument Compatibility

ABI 7000, 7300, 7700, 7900, 7900HT, 7900HT FAST, StepOne™, StepOne™ Plus (see product selection table) each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.

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