Real-Time PCR Mixes

Bioline SensiFAST Lyo-Ready No-ROX Mix, 10 x 10ml (BIO-BIO-11060)


A novel SensiFAST mix that can be lyophilized with assay-specific primers and probes to produce ambient temperature stable qPCR master mixes that give outstanding assay reproducibility, sensitivity and robustness following rehydration.

Product Highlights

Glycerol-free - ideal for preparation of custom lyophilized qPCR master mixes for improved convenience and extended room-temperature stability
Reproducible - consistent results between technical replicates for increased confidence in results
Robust - reliable, accurate detection of DNA and cDNA targets from a broad range of sample types
Sensitive - reliable quantification of low abundance targets and scarce samples
Fast - delivers reproducible, accurate assay results in as little as 30 minutes
Efficient - excellent performance in multiplex assays
Specific - antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability

Delivery time: 2 Weeks
Delivery times may vary
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The SensiFAST™ Lyo-ready No-ROX Mix is a glycerol-free qPCR mix that has been developed for fast, highly reproducible real-time PCR and has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX.

A combination of the latest advances in buffer chemistry, PCR enhancers and lyo-excipients, together with an antibody-mediated hot-start DNA polymerase, ensures that the SensiFAST Lyo-ready No-ROX Mix produces reliable assay results under fast thermal cycling conditions and when multiplexing of high- and low-copy targets.

SensiFAST Lyo-Ready No-ROX Mix together with assay-specific primers and probes can be lyophilized to produce ambient temperature stable qPCR master mixes. Millisecond rehydration times make SensiFAST Lyo-Ready No-ROX Mix ideal for automated, high throughput systems.


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Diagnostic test development
Pathogen detection
Drug therapy efficacy
DNA / RNA target detection
Copy number variation (CNV) analysis
Gene expression analysis

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