Nucleic Acid Isolation

Bioline SureClean

Features

• Column-free PCR clean-up
• Post-PCR recovery up to 98%
• Cost-effective and simple protocol
• Isolated products are suitable for downstream applications


Delivery time: 2 Weeks
Delivery times may vary
Request Info
Request Quote
Send To a Friend
Print

Product Description
SureClean is a novel, inexpensive solution, which provides a column-free method for nucleic acid purification. Using a simple and rapid procedure, SureClean can be used to purify or concentrate DNA or dsRNA from PCR reactions or any enzymatic digests. This method is easy to follow, combining convenience, speed and excellent recovery rates.

A very straightforward protocol allows the precipitation of nucleic acids ≥75bp with up to 98% recovery of the original sample, without the need for organic solvents, glass milk or expensive spin columns. SureClean purifies nucleic acids without the use of chaotropic salts (which often contribute to denaturation of the DNA duplex). SureClean enables the researcher to re-suspend the cleaned-up nucleic acids in any buffer and volume of choice, thus permitting the purification process to be tailored specifically to suit downstream experiments.

SureClean is compatible with all downstream applications, such as cloning and sequencing.

Applications
Removes primers, non-specifics, dNTPs and enzymes
DNA or dsRNA purification or concentration
Buffer exchange

Optional Pink Co-Precipitant
SureClean Plus incorporates a pink co-precipitant that offers the distinct advantage of easy visualization of the purified pellet. Co-precipitant Pink is a synthetic polyacrylamide, designed to aid recovery of nucleic acids. Co-precipitant Pink does not contain any detectable amounts of nucleic acids, and is suitable for use in standard PCR, RT-PCR, and other enzymatic reactions.

Storage Conditions
SureClean solution can be stored at room temperature for 12 months. Do not freeze. Co-precipitant Pink can be stored at +4°C for up to 6 months or at -20°C for 12 months. Avoid exposure to light.

Note
Nucleic acids purified with SureClean Plus are not suitable for spectral determination of nucleic acids within the 260nm - 280nm range. Should you wish to carry out such experiments, the use of SureClean is recommended.

1. Becher, R. et al. BMC Genomics 12:52 (2011).
2. Mancini, E. et al. BMC Evol. Biol. 11, 292, (2011).
3. Vasovcak, P. et al. PLoS One 6(8), e24114 (2011).
4. Uil, T.G. et al. NAR 39, e30 (2011).
5. Tourlomousis, P. et al. Microb. Eco. 59(4), 776-786 (2010).
6. Drögemöller, B.I. et al. Pharmacogenomics 11(8), 1095-1103 (2010).
7. Ferguson, R.M.W. et al. J. Appl. Microbiol. 109(3), 851–862 (2010).
8. Bilek, N. et al. J. Bacteriol., 191, 1878-1890 (2009).
9. Horbach, R. et al. Plant Cell, 21, 3379-3396 begin_of_the_skype_highlighting              3379-3396      end_of_the_skype_highlighting (2009).
10. Matheson, L.S., et al. Int. Immunol. 21, 957-966 (2009).
11. Aldhoun, J.A., et al. Parasitol. Int. 58(3), 314-317 (2009).
12. Ward, E. Meth. Mol. Biol. 508, 1-13 (2009).
13. Pecyna, M.J., et al. Appl. Microbiol. Biotechnol. 84(5), 885-897 (2009).
14. Liapis, E., et al. NAR 36(18), 5933-5945 (2008).
15. Huang, D., et al. J. Mol. Evol. 66(2), 167-174 (2008).
16. Shefer, K., et al. Mol. Cell. Biol. 27(6), 2130-2143 (2007).
17. Barberi, M., et al. Reprod. 134 281-292 (2007).
18. D’Amelio, S., et al. Parasitol. 134, 1041-1051 (2007).

No related products found.