Nanophotometer

Implen OD600 DiluPhotometer (IMP-OD600)

Implen OD600 DiluPhotometer

Features

The OD600 DiluPhotometer™ has been especially designed for the spectrophotometrical analysis of bacterial and yeast growth rates and the quantification of proteins at a wavelength nearby 600 nm (e.g. Bradford). Combining OD600 DiluPhotometer™ with Implen's DiluCell™ 10 or DiluCell™ 20 facilitates measurements with low sample volumes of 200 µl and 100 µl, respectively and a virtual sample dilution of either factor 10 or 20.
The instrument is battery operated and can be easily transported for usage in incubation cabinets and under anaerobic conditions. The batteries are recharged automatically by connecting to the mains and last for almost 1 month when fully charged. A 600nm LED source in combination with a fiber optic is used to obtain the optical density measurement. Spillages can be easily wiped from the even surface, and the cell compartment area can be rinsed with, formaldehyde or ethylene oxide to sterilize it.


Delivery time: 4-6 Weeks
Delivery times may vary
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• Small, portable spectrophotometer for quantifications at 590 to 610 nm
Configured for bacterial growth rates and protein assays (Bradford)
• GLP/GMP ready with DiluCell™
Automatic sample dilution of Factor 10 and 20, Small volume requirement 200 µl or 100 µl
• Fast and easy handling
Measurement in less than 2 seconds
• Battery operated
Unlimited mobility, ideal for usage in incubation cabinets and under anaerobic conditions
• Small sample volumes
100 µl or 200 µl
• Built-in Memory for data storage
Storage of up to 99 measurements
• Data Output via RS232 interface
Submit data on a computer or print data with OD600™ thermal printer locally
• Maintenance free setup
No moving parts, no recalibration necessary

Wavelength

600nm

Bandwidth

40nm

Range

Optical Density - 0.3A to 1.99A

Accuracy

< ±0.05A at 1A using Neutral Density Filters

Repeatability

±0.02A at 1A

Controls

6 button - power, blank, sample, memory, re-set, recall/print

Optical height

8.5 mm

Cuvette holder

Fixed with drain hole. Accepts DiluCell™ 10, DiluCell™ 20, 10 mm pathlength semi micro and macro cuvettes or 14 – 16 mm round tubes.

Output

RS232, USB

Memory

99 readings

Display

Custom LCD

Power requirements

External power adaptor (110 to 220 V, 50/60 Hz, 20 VA) or internal rechargeable NiMH battery

Approximate dimensions

180 x 150 x 60mm

Weight

0.6kg

 

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Measuring Cell Density
The stage of growth of a bacterial culture needs to be monitored to ensure that the cells are harvested at the optimum point for the greatest density of live cells. An exemplary growth curve is given. Cells should be harvested towards the end of the log phase. The optical density of the sample indicates when this point has been reached. This value varies dependent on the cells being grown. Routinely the cells are grown until the absorbance at 600 nm (known as OD 600) reaches approximately 0.4 prior to induction or harvesting. A linear relationship exists between cell number (density) and OD 600 up to approx. 0.6. Combining OD600 DiluPhotometer™ with Implen's DiluCell™ 10 or DiluCell™ 20 allows the quantification of bacterial and yeast cultures with low sample volumes of 200 µl and 100 µl, respectively without prior dilution. The cultures are virtually diluted with either factor 10 or 20 and the measurements can be taken directly at the laboratory area where cells are cultured.

Protein Quantification with Bradford Assay
Bradford Assay is a rapid and accurate method used to determine the total protein concentration of a sample by quantifying the binding of a dye, Coomassie Brilliant Blue G-250, to an unknown protein. The assay is based on the observation that the absorbance maximum for an acidic solution of Coomassie Brilliant Blue G-250 shifts from 465 nm to 595 nm when binding to protein occurs.
By comparing this binding to that of different, known concentrations of a standard protein, the concentration of the unknown protein can be calculated. Standard curves with bovine serum albumin (BSA) are given as an example.

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