Sequencing

Bioline JetSeq DNA Library Preparation Kit, 16 reactions (BIO-BIO-68025)

Bioline JetSeq DNA Library Preparation Kit, 16 reactions

Features

  • Low input: end-repair, A-tailing and ligation combined in the same tube, thereby eliminating cleanup steps and improving sample yield
  • Improved confidence: simpler automatable protocol with fewer steps for reducing the risk of sample loss, offering greater peace-of-mind
  • Increased speed: library preparation in less than 3 hours for reduced time-to-results and increased sample throughput
  • Highly efficient: reaction buffer pre-optimized to provide maximum reaction efficiency and highest conversion rates
  • Improved quality: reliable library preparation from even the most challenging samples providing maximum coverage
  • Maximum convenience: sequencing adaptors, enabling indexing of up to 16 samples, already included in the kit

The success of next-generation sequencing is dependent upon the precise and accurate processing of the input DNA. This requires high quality library preparation from sheared DNA and relies on a coordinated series of standard molecular biology reactions whilst maintaining high yields during the intermediate purification steps.

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Instrument Compatibility

JetSeq DNA Library Preparation Kit is compatible with the Illumina MiSeq, NextSeq and HiSeq instruments.

Description

The success of next-generation sequencing is partly dependent upon the precise and accurate processing of the input DNA. High-quality library preparation from sheared DNA requires efficient processing during a series of molecular biology reactions and good recovery during the intermediate purification steps. Bioline has developed an optimized, single-tube library construction method (fig. 1) that enables fast and highly efficient adaptor ligated library construction from a range of sample types and inputs.

The combination of end repair and A-tailing followed by direct ligation of the adaptors reduces the hands-on time, making the process faster and easier to use. Optimized end-repair, A-tailing and ligation steps and the reduction in cleanup steps also offer significant improvement in the yield and quality of sequence-ready libraries, reducing the amount of DNA required.

The JetSeq DNA Library Preparation Kit is highly efficient at converting DNA into an adapter-ligated library resulting in higher sequence coverage. The kit contains all of the enzymes and buffers necessary for highly-efficient conversion of the input DNA to a sequencable, adapter-ligated library (fig. 2).

The JetSeq DNA Library Preparation Kit uses a highly efficient polymerase, together with an optimized buffer formulation to ensure uniform amplification of all genomic regions including those that contain highly variable GC content, thereby reducing bias and ensuring even coverage in subsequent sequencing reactions, resulting in exceptional sequence quality scores even 150 bases into the read (fig. 3), giving greater coverage and reducing the possibility of sequencing gaps.

 

Cat No. BIO-68025
ER Buffer 160 µl
ER Enzyme Mix 96 µl
Ligation buffer 48 µl
Adaptor A 80 µl
Adaptor B 80 µl
Ligase 32 µl
PCR Buffer 80 µl
Primer Mix 80 µl
DNA Polymerase 32 µl
PCR Buffer 80 µl
Primer 16 µl
DNA Polymerase 32 µl
Nuclease Free Water 1.8 ml
Index 1 - 16 20 µl

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